Thyroid Hormone Receptor Nuclear Export

Multiple Novel Signals Mediate Thyroid Hormone Receptor Nuclear Import and Export*

Thyroid hormone receptor (TR) is a member of the nuclear receptor superfamily that shuttles between the cytosol and nucleus. The fine balance between nuclear import and export of TR has emerged as a critical control point for modulating thyroid hormone-responsive gene expression; however, sequence motifs of TR that mediate shuttling are not fully defined. Here, we characterized multiple signals...

Retinoid X Receptor Regulates Nur77/Thyroid Hormone Receptor 3-Dependent Apoptosis by Modulating Its Nuclear Export and Mitochondrial Targeting

Thyroid hormone nuclear receptor. Evidence for multimeric organization in chromatin.

Using cultured GHI cells, we have studied the organization in chromatin of the thyroid hormone nuclear receptor. We have previously shown that the receptor protein can be extracted from nuclei with 0.4 M KC1 as a 3.8 S species, and that micrococcal nuclease excises the receptor as an abundant 6.5 S form and a less abundant 12.5 S form (Samuels, H. H., Stanley, F., Casanova, J., and Shao, T. C. ...

Affinity labeling of rat liver thyroid hormone nuclear receptor.

The thyroid hormone receptor from rat liver nuclei has been covalently labeled with the N-bromoacetyl derivatives of L-thyroxine (T4) and 3,3',5-triiodo-L-thyronine (T3). Displacement binding studies showed that, in the presence of 100-fold molar excess of unlabeled N-bromoacetyl-T3 or T4, binding of [125I]T3 or [125I]T4 was nearly totally inhibited. Heat inactivation of the receptor (55 degree...

Interactions of the nuclear thyroid hormone receptor with core histones.

These studies concern the interactions of the rat liver thyroid hormone nuclear receptor with histones and factors influencing the receptor's assay and stability. Heating certain crude receptor preparations at 50 degrees C produces a selective loss of triiodothyronine (T3) but not thyroxine (T4) binding activity, whereas, with more purified preparations, such heating decreases both T3 and T4 bi...